LACTATE DEHYDROGENASE AND ITS ISOZYMES IN HUMAN EJACULATE

Amino- and carboxyl-terminal analyses of hepatoma lactate dehydrogenase isozymes.

The M4 isozyme of lactate dehydrogenase was purified to homogeneity from normal rat liver and from two Morris hepatomas (7777 and 7793). Amino-terminal analyses with fluorodinitrobenzene failed to detect the presence of free amino-terminal residues in each enzyme studied. Each enzyme contained between 3.7 and 4.1 moles of protein-bound acetyl groups per mole of enzyme. The amino-terminal peptid...

Genetic Evidence for the Subunit Structure of Lactate Dehydrogenase Isozymes.

The lactate dehydrogenases commonly occur in from one to five molecular forms (isozymes) in the various tissues of an animal species.' By means of zone electrophoresis, characteristic patterns of these isozymes may be demonstrated in each tissue. In adult animals, most tissues contain all five isozymes but in different proportions. Usually, heart muscle exhibits principally the first, or most n...

Isozymes of lactic dehydrogenase in human tissues.

The heterogeneity of lactic dehydrogenase (LDH) activity in human serum and erythrocytes has been observed in several laboratories (1-7). If each of the electrophoretically-distinguishable enzymes in serum with LDH activity were derived from different tissues, in disease states involving a single organ the normal pattern of serum LDH activity would be altered according to the type of LDH releas...

Isozymes of lactate dehydrogenase (LDH) in skeletal tissues of the embryonic and newly hatched chick.

Effect of infection and endotoxicosis on plasma lactate dehydrogenase isozymes in white rats.

Vertical slab electrophoresis in polyacrylamide gels was used to monitor changes in lactate dehydrogenase (LDH) isozymes in plasma of white rats during bacterial infection and endotoxin poisoning. Peritoneal infection with Francisella tularensis and Salmonella typhimurium and administration of S. typhimurium endotoxin stimulated significant increases in plasma LDH-5. Rates of change in enzyme a...